Search results for "sample presentation"

Sample preparation and analysis of gangliosides and phospholipids from plasma by liquid chromatography coupled to mass spectrometry

2016

Gangliosides (GG) and phospholipids (PL) are two classes of complex and polar lipids exhibiting a wide structural heterogeneity. LC/MS offers an access to this huge diversity but also faces some challenges. Efficient extraction and purification sample methods are thus required to perform accurate and reliable analyses. To this end, GG and PL were extracted from human plasma with three solvent mixtures (CHCl3/MeOH/H2O) of increasing polarity, in comparison with the reference Folch method. GG were further purified with a Phree Phospholipid Removal column, whose sorbent is designed to selectively remove PL. GG and PL classes were then separated under HILIC conditions before analysis using a Qq…